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2 edition of uptake of L-Triiodothyronine by isolated rat hepatocytes found in the catalog.

uptake of L-Triiodothyronine by isolated rat hepatocytes

Anne Jane Devlin

uptake of L-Triiodothyronine by isolated rat hepatocytes

by Anne Jane Devlin

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Published by University ofBirmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D) - University of Birmingham, Dept of Medicine, 1992.

Statementby Anne Jane Devlin.
ID Numbers
Open LibraryOL20860716M

@article{osti_, title = {Stabilization of glucocorticoid receptors in isolated rat hepatocytes by radioprotectants}, author = {Karle, J.M. and Ridder, W.E. and Wright, N. and Olmeda, R. and Nielsen, C.J.}, abstractNote = {Previous work has shown that glucocorticoid receptors in rat liver homogenate can be stabilized by the addition of MoO/sub 4/ plus the sulfhydryl-containing compounds. The authors studied the hepatic uptake of carrier-free Ga using isolated rat hepatocytes and perfused livers. The results indicate that: (a) the liver can take up Ga and secrete it into the bile, even in the absence of transferrin; (b) transferrin inhibits hepatic uptake of Ga and its biliary excretion; and (c) iron deficiency markedly.

Primary rat hepatocytes were isolated from female Sprague Dawley rats (Envigo) by collagenase perfusion and were cultured in William’s E Medium (5% FBS) as described by previously (Shen et al., ). All animals received humane care in accordance with the guidelines established by the American Association for the Accreditation of Laboratory.   Our ultimate goal was to co-culture primary hepatocytes and LSECs isolated from the same rat. This required sacrificing two rats with a few days' interval. In order to test our co-culture idea without having to sacrifice many rats we sought to use an established endothelial cell line and chose BAECs because it was readily available to us.

According to studies in the isolated perfused rat liver (35, 36) as well as in isolated rat hepatocytes (22, 41, 46), it appears that ∼75% of the uptake of taurocholic acid (TCA) is Na +-dependent. A Na + -dependent bile acid transporter Na + /taurocholate cotransporting polypeptide (ntcp) was subsequently expression cloned from rat liver. *Andrae U, Schwarz LR. Induction of DNA repair synthesis in isolated rat hepatocytes by 5-diazouracil and other DNA damaging compounds. Cancer Lett *Arai M, Aoki Y, Nakanishi K, et al. Long-term experiment of maximal noncarcinogenic dose of dimethylnitrosamine for carcinogenesis in rats. Gann


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Uptake of L-Triiodothyronine by isolated rat hepatocytes by Anne Jane Devlin Download PDF EPUB FB2

1. Rat liver cells obtained by dispersion with collagenase were used to investigate the mode of entry of L-tri-iodothyronine into the cell. The hormone was taken up very rapidly at 23 degrees C; the linear phase of uptake lasted for up to approx. Eric P. Krenning, Roel Docter, Martine Polhuys, Bert F.

Bernard, Theo J. Visser, Jan A. Mol, Georg Hennemann, Carrier-Mediated Transport of Thyroid Hormone (TH) into Rat Hepatocytes is Rate Limiting in Total Cellular Uptake and Metabolism, Frontiers in Thyroidology, / Cited by:   TOXICOLOGY AND APPLIED PHARMACOL () Lidocaine Uptake in Isolated Rat Hepatocytes and Effects of dl-Propranololl CHI-PO CHEN,2 VAN T.

VU, AND STEVEN D. COHEN Section of Pharmacology and Toxicology, School of Pharmacy, The University of Connecticut, Storrs, Connecticut Received Janu ; accepted May 7, Lidocaine Uptake in Isolated Rat Hepatocytes Cited by: Abstract We have demonstrated (Saito et al., ) that in isolated rat hepatocytes, L-tryptophan (Trp) uptake occurs via at least two saturable transport systems [one high-affinity (Km = 14 μM) and one low-affinity (Km = mM)] and one non-saturable by: 2.

The transport of [Cr]chromate into human erythrocytes and isolated rat hepatocytes has been investigated. It was found that uptake in both cell types could be inhibited by the established anion carrier inhibitor 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid.

The uptake Cited by: Studies of the uptake of dieldrin, lindane, and DDT by isolated rat hepatocytes in Krebs-Henseleit salt solution containing % bovine serum albumin have shown that the partition equilibrium of these hydrophobic organochlorine compounds is established very rapidly between the hepatocytes and the extracellular medium by reversible uptake and release processes.

Hepatocellular uptake of the endogenous sulfate ester estrone sulfate was characterized by use of isolated liver cells: (1) Uptake was saturable with an apparent K M of μM and a V max of nmol/mg protein × min.

(2) Uptake was inhibited by the sulfhydryl (SH-) poisons mersalyl and dinitrofluorobenzene. (3) Taurocholate and estrone sulfate competed for uptake. Development of enzymatic methods for isolated hepatocyte preparation. Principles of cell separation.

Separation of cells from solid organs other than liver. Advantages of the isolated hepatocyte preparation. High-yield preparation of isolated hepatocytes from rat liver. Introduction. One-step method. The transport mechanism of pravastatin, a new cholesterol-lowering drug, was compared in vitro with rat hepatocyte primary culture and mouse skin fibroblasts (L-cells).

The uptake of 14 C-labeled pravastatin by cultured hepatocytes was temperature- and dose-dependent. The temperature-dependent uptake as a function of [14 C]pravastatin concentration showed saturation kinetics with K. Abstract. Previous studies have demonstrated that hepatic uptake of bilirubin and bile acids show the characteristic features of a carrier-mediated transport and have suggested that maturation of this system may in part account for the developmental immaturity of the hepatic excretory system (Scharschmidt et al, ; Iga and Klaassen, ; Suchy and Balistreri, ).

Endocrinology8. Eckel J, Rao GS, Rao ML, et al: Uptake of L-triiodothyronine by isolated rat liver cells. Biochem J9. Krenning E, Docter R, Bernard B, et al; Characteristics of active transport of thyroid hormone into rat hepatocytes.

Biochim Biophys Acta  The Impact of Solute Carrier (SLC) Drug Uptake Transporter Loss in Human and Rat Cryopreserved Hepatocytes on Clearance Predictions.

Drug Metabolism and Disposition42 (3), DOI: /dmd   This mode of uptake of tri-iodothyronine by human erythrocytes is in sharp contrast with that of rat hepatocytes, which uptake system is energy-dependent and ouabain-sensitive [Krenning, Docter, Bernard, Visser & Hennemann () FEBS Lett.

91, –; Krenning, Docter, Bernard, Visser & Hennemann () FEBS Lett. –]. Endocytosis and degradation of bovine apo- and holo-lactoferrin by isolated rat hepatocytes are mediated by recycling calcium-dependent binding sites.

Biochemistry32 (49), DOI: /bia Judith Yannariello-Brown, Paul H. Weigel. Mooradian AD, Schwartz HL, Mariash CN, Oppenheimer JH. Transcellular and transnuclear transport of 3,5,3'-triiodothyronine in isolated hepatocytes. Endocrinology. Dec; (6)– Centanni M, Robbins J.

Role of sodium in thyroid hormone uptake by rat skeletal muscle. J Clin Invest. Oct; 80 (4)– [PMC free article]. Isolated rat hepatocyes bind, internalize, and degrade bovine lactoferrin (Lf) via high-affinity Ca2+-dependent sites [rat hepatocytes.

The coimmunoprecipitation of IR and GLUT2 has been reported in rat hepatocytes and more recently in immortalized neonatal hepatocytes. Furthermore, the lack of IR in neonatal hepatocytes resulted in a significant decrease in basal glucose uptake, supporting the hypothesis that IR and GLUT2 form a receptor-transporter complex in hepatocytes.

Rapid and direct stimulation of hepatic gluconeogenesis by L-triiodothyronine (T3) in the isolated-perfused rat liver. Life Sci. Sep 8; 27 (10)– Dembri A.

Effets métaboliques de la 3,5-diméthyl-3'-isopropyl-L-thyronine (DIMIT) et de la 3,5,3'-triiodo-L-thyronine (T3) dans le cerveau et le foie de rat hypothyroïdien. Hepatocytes sequestered sucrose at an initial rate of approximately 10%/h, which is threefold higher than the estimated rate of autophagic-lysosomal protein degradation.

Almost one-third would appear to represent mitochondrial fluid uptake; the rest was nearly completely and specifically inhibited by 3-methyladenine (3MA) and can be regarded as.

L-triiodothyronine acutely increases Ca2+ uptake in the isolated, perfused rat heart. Changes in L-type Ca2+ channels and beta-receptors during short- and long-term hyper-. As an experimental system, we have used chick embryo hepatocytes, isolated at two different stages of development.

It is known that the liver is a pivotal target for the genomic effects of thyroid hormones, and about 5% of hepatic genes are under thyroid hormone control (30), but little information is available on the presence and the.The Impact of Solute Carrier (SLC) Drug Uptake Transporter Loss in Human and Rat Cryopreserved Hepatocytes on Clearance Predictions.

Drug Metabolism and Disposition42 (3), DOI: /dmdThe sugars [14C]sucrose, [14C]lactose and [3H]raffinose have been loaded into isolated rat hepatocytes by means of electropermeabilisation, and used as probes of autophagic-lysosomal function.